"Use of Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) and ITS2 PCR assays for differentiation of populations and putative sibling species of Anopheles fluviatilis (Diptera: Culicidae) in Iran"

"Use of Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) and ITS2 PCR assays for differentiation of populations and putative sibling species of Anopheles fluviatilis (Diptera: Culicidae) in Iran"

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Anopheles fluviatilis complex is tennessee titans dog bandana known to be a vector of malaria in Iran.Since mosquitoes of this species cover a wide geographical range in Iran, they might have evolved into different separated populations.Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) assay was used to differentiate geographic populations of this species.DNA was extracted from individual mosquitoes from 8 localities in 4 south and southeast provinces and amplified in PCR reactions using 18 single primers of arbitrary nucleotide sequence.Results of RAPD-PCR showed that Kazeroun populations could simply be differentiated from other populations using a diagnostic fragment amplified with primer UBC-306.

But other populations could not be differentiated either visually or by means of statistical analysis.Moreover ITS2 fragments of some selected specimens were amplified using a pair of universal primer and sequenced as a key standard for detection of putative sibling species.Sequence analysis of the ITS2 fragments revealed a very high (100%) ngetikin homology among the populations.These findings are crucial in epidemiological studies concerning relatedness of geographic populations and vector movement in the region.Results of RAPD-PCR and ITS2 analysis suggest that this taxon in Iran comprises of only one species with a low genetic variation among geographic populations.